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Sections of these blocks were obtained as previously described [ 2].
Selected areas from tissue blocks were obtained as follows: Cryosections (20 μm) were mounted on PEN slides (P.A.L.M. Microlaser, Bernried, Germany).
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A hyperbranched, highly hydrophilic polyglycerol block is obtained as the polar segment of the structure.
Finally, the motion vectors for that block are obtained as the difference between the position in t and the positions in the next and the previous image.
The forward and backward MVs for each block are obtained as: begin{aligned} mathrm{MV}_{B}^{n} = frac{-mathrm{MV}^n}{2}, quad mathrm{MV}_{F}^n = frac{mathrm{MV}^n}{2}.
After cleavage from the solid support the lysine dendrimer/linker building block was obtained as Boc-protected carboxylic acid 9 (Scheme 1).
Tumor blocks were obtained for 647 probands.
Tissue blocks were obtained on 36 patients.
Formalin-fixed, paraffin-embedded tissue blocks were obtained by xenograft.
Histological sections and paraffin blocks were obtained from the Hospital Department of Pathology.
Formalin-fixed, paraffin-embedded brain blocks were obtained for immunohistochemistry.
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