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Tissue blocks were not available for five samples.
Of these, 177 women were excluded because their tumor blocks were not available from the Hawaii Tumor Registry (HTR).
In addition, 25.3% of the tumour blocks were not available or had been discarded by the pathology laboratories.
Those with invasive cancer and those for whom diagnostic core biopsy blocks or surgical blocks were not available were excluded.
Importantly, single-fiber analysis in all patients, with the exception of patients 1 and 6 for whom orientated muscle blocks were not available, demonstrated a statistically significant segregation of mutation load with biochemical deficiency (Fig. 3).
Our study has a number of limitations: (1) The number of controls who had both LC and spinal cord tissue available was limited, since spinal cord tissue was usually removed at post mortem from controls only when clinically indicated, and LC-containing blocks were not available from all potential controls.
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Currently, information about the functions and evolutionary origins of these conserved blocks is not available.
The finest spatial resolution for which hospital discharge data were available was the 5-digit postal ZIP code of the patient's residence; the patient's street address, 9-digit ZIP code, or census block were not available.
Although the precise age of each excluded block was not available, it is reasonable to assume that the samples with unacceptable RT-PCR data may have been from the oldest blocks [ 5].
Secondly a diagnosis of "probable" CGH was only possible as diagnostic injection block procedures were not available, hence some subjects may have been misclassified.
Because population estimates for census block groups were not available for intercensal years, we multiplied the 2000 population counts by five and three, respectively, to estimate the total population at risk for the 5-year period of incidence and 3-year period of mortality.
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