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Slides were loaded on a DAKO autostainer and blocked with serum-free protein block solution (Dako #X0909) which was applied for 5 minutes and then FAK primary antibody (Millipore #05-537) wappliedied for one hour.
Protein lysates were loaded on 10 12.5% SDS-PAGE gels, transferred onto PVDF membranes and blocked with 5% BSA in PBST for 1 h.
After blocking with 2 % BSA in PBS at 37 °C for 1 h, the samples were loaded on the plates and incubated overnight at 4 °C.
Unboiled samples were loaded on the gel.
were loaded on the gel.
The hunt had been halted temporarily last week, after Sea Shepherd boats blocked the stern of Japan's mother ship, preventing any harpooned whales from being loaded on.
The Sea Shepherd Conservation Society says one of its boats has been blocking the main ship's stern loading ramp, preventing any harpooned whales from being loaded on to the ship.
There the sacks are loaded on planes.
Paulette was loaded on something.
After blocking, slides were loaded to the autostainer.
While the game is loading on DVD, for example, pertinent blocks are placed closer together to speed load times – and the PS3 writes those blocks a few times on the disk to make things even faster – while the HD drive spins around madly like a dervish as it seeks and reads.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com