Like Cassandra, I liked to "capture" the people and things around me with words and pictures, and these days I often think of Cassandra's dad with his worst-ever case of writers' block, being left in a dungeon to write!
The bone cement block was left in place in animals with S. epidermidis inoculum in order to mimic a foreign-body-associated infection, while it was removed in S. aureus animals.
The cement block was left in place in animals with S. epidermidis inoculation because the persistence of culture-positive S. epidermidis bone infection requires the presence of a foreign body [17, 19].
Five methodological samples were excluded based on 260/280 nm or 260/230 nm ratio and an additional frozen sample was excluded because no comparison FFPE blocks were left (Table S1 in Additional file 1).
The largest number of critics were residents and workers in Chinatown whose blocks were left out of the rezoning plan and who raised concerns that businesses hurt by the changes would be pushed into their neighborhood.
The blocks then were left in a 1 1 solution of propylene oxide EM-bed 812 (EM Sciences) oxide EM-bed
Just yards away, the torrent ripped away foundations from a block of flats, which were left in danger of collapse.
After the introduction of the 18-column table, the group IB elements were moved to their current position in the d-block, while alkali metals were left in group IA.
After three additional washes in PBS, cells were left in blocking solution (PBS supplemented with 1% horse serum and 0.1% Triton) for 1 h at RT. Cells were incubated with primary antibodies o/n at 4 °C.
For detection of the antigens with fluorescent antibodies, the protocol was performed as follows: sections were left in blocking solution (PBS supplemented with 1% horse serum and 0.1% Triton) for 1 h at RT and then incubated overnight at 4 °C with primary antibodies diluted in blocking solution.
