Sentence examples for blocks were immersed in from inspiring English sources
The 20 bone blocks were immersed in a solution of 4%% formaldehyde, dehydrated in ethanol, and embedded in methyl methacrylate.
The tissue blocks were immersed in the chamber filled with 100% dibenzyl-ether for the volume-imaging procedure.
The fixed brain blocks were immersed in 30% sucrose dissolved in 0.1 M PB until they sank down to the bottom.
Tissue blocks were immersed in 2.5% glutaraldehyde fixative/0.1 M sodium cacodylate buffer for one hour and then rinsed 0.1 M sodium cacodylate buffer three times ten minutes each.
Fixed tissue blocks were immersed in 2.3 M sucrose for 20 min at room temperature (18 24°C), mounted on a specimen holder and snap-frozen in liquid nitrogen.
For PrPSc detection, formalin fixed tissue blocks were immersed in 98% formic acid for 1 hour prior to paraffin embedding, and slides were autoclaved at 121°C for 5 minutes in 0.1 M citrate buffer pH 6.0 and treated with trypsin (Difco) at 37°C for 5 minutes.
The fixed brain blocks were immersed into 30% sucrose in 0.1 M PB until they sank down on the bottom.
A silicone coating was applied to the exterior, and the block was immersed in a tank of water to which hydrazine was added to provide a chemically reducing barrier.
The block was immersed in a mixture of 30%sucrose/10%% formalin in PBS for 2 hr and then stored in 10% sucrose in PBS at 4°C for up to 2 weeks.
After discarding the soaking buffer, the block was immersed in 100 μl of digestion buffer containing 20 U of I-PpoI (Promega) and incubated for 1 1.5 hours at 37°C.
Before sectioning, such blocks were immersed overnight in 30% phosphate-buffered sucrose at 4 °C, then 50-μm sections were cut with a freezing microtome and sections collected free floating in PBS.
