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Haplotype blocks were identified using the 4 gamete test as implemented in Haploview.
Haplotype blocks were identified using Haploview v4.2 [ 29] following the methods described in [ 30].
Haplotype blocks were identified using genotyping data from control individuals as well as using HapMap data from the CEPH cohort [ 61] using the Haploview [ 62, 63] software.
Genes that are located within the haplotype blocks were identified using Ensembl version 69 [ 64] gene annotations as provided by Ensembl BioMart [ 65] in the Rn4 assembly.
The genes in the soybean genome that have retained paralogs within syntenic blocks were identified using BLAST (Altschul et al. 1990), DAGChainer (Haas et al. 2004), and selection of gene pairs from syntenic blocks with average Ks values between 0.03 and 0.60; the full list of such genes has been previously published (Anderson et al. 2014).
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After applying preprocessing techniques (median filtering and binarization), the initial blocks are identified using the connected component analysis (CCA) method.
The LD block was identified using the default algorithm implemented in the define blocks function of Haploview 4.2 software [ 50].
As a next step, all blocked reactions present in Recon_1_decomp were identified using flux variability analysis (FVA) [ 27] as reactions unable to carry flux defined by |Vmax, i | ≤ 10-5 mmol/gdw/hr and |Vmin, i |≤ 10-5 mmol/gdw/hr for all i reactions in the network.
Colinear blocks containing at least five genes were identified using MCScanX with the parameters: -s 3 -m 556.
To distinguish those LSG non-LSG pairs which have originated from LSG non-LSG segmentally dupairsted blocks, paralogous syntelogs in Arabidopsis thaliana whichidentified using SynMap (powered by DAGchaveer [ 34]) part originatede package [ 35].
Testicle derived samples were hybridised using a balanced block design and significantly affected genes were identified using linear models (Limma).
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