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Haplotype blocks were generated using the default algorithm based on methods established by Gabriel et al. (2002).
Cell blocks were generated using synchronized G1-phase populations of HeLa cells and 5 µm sections were mounted onto a glass slide without any staining as described in Section 6.3.1.
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First, a block warping procedure is applied where two warped blocks are generated using two sets of perspective transform parameters, derived (by solving (2)) from each of the perspective transforms T ˙ b and T ˙ f, one for each up-sampled reference frame (backward and forward).
Given a coefficient vector B4 ∈ ℜ P 4×1 (all zeros but the first ten), the probability of disease π was computed by using (20) π = exp X 4 B 4 1 + exp X 4 B 4. Then, the binary class label block was generated using the Bernoulli distribution with the probability π.
MSAs in block format were generated using WAVIS server (http://wavis.img.cas.cz) [55].
Haplotype blocks and LD structure were generated using Haploview [ 23].
Paraffin blocks of RHEK1 and RHEK1/LMP1 cells were generated using a commercially available Cell Block Preparation System (Shandon Cytoblock, Thermo Scientific, Astmoor, UK).
Expression vectors for the negative control (miR-control) and the miRNAs were generated using BLOCK-iT™ PolIImiR RNAi Expression Vector Kits (Invitrogen).
38, 44 Scientists at Medarex, led by Alan Korman, then developed human monoclonal antibodies that block human CTLA-4, which were generated using a transgenic mouse comprising human immunoglobulin genes.
RACK1 RNAi cells were generated using the BLOCK-iT™ Inducible H1 Lentiviral RNAi System (Invitrogen) according to the manufacturer's instructions and then cultivated as described above.
PCR products were generated using a block thermocycler as described previously.
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