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These blocks were fixed by means of threaded screws to the bottom of the acrylic resin plate.
Hippocampal tissue blocks were fixed by immersion in 4% paraformaldehyde and 2% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4 overnight, and postfixed in 1% osmium tetroxide for 2 hours.
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The composition of these two-material building blocks is fixed by volume fraction Figure 1 Two kinds of heat conduction building blocks considered in this study: (a) cylinder--cylinder building block; (b) rectangular-prism--cylinder building block.
Then lung blocks were fixed, embedded, cut and stained.
An additional seven GNGs corresponding to blocked reactions were fixed by this method but were not added to the list of proposed model modifications due a lack of experimental validation (see Additional file 2 for uncorroborated model changes).
Brain blocks including the mesencephalon were fixed by immersion in phosphate-buffered 4% paraformaldehyde for 24 h and cryoprotected for 48 h in a solution containing 20% of glycerin and 2% DMSO in 0.125 M phosphate buffer (PB), pH 7.4.
In brief, small blocks of muscle tissue (2*2*2 mm) from both legs were fixed by glutaraldehyde and post-fixed by osmiumtetroxide and epon embedded.
We could only fix one GSL inconsistency by modifying the biomass equation: KRE6 (YPR159W) and SKN1 (YGR143W) that provide isozymes for the blocked reaction 16GS (1,6 β-glucan synthase) were fixed by including 16BDglcn (1,6 β-D-glucan) a root no-consumption metabolite in the biomass equation as a reactant.
The cells were fixed by 3% paraformaldehyde and blocked against nonspecific binding by incubation in 3% goat serum containing 0.05% Triton-X 100.
As with pure blocks, the order of mixed blocks was fixed: neutral-emotional-neutral-emotional.
The sections were fixed by acetone, and then incubated with 0.1% BSA to block the activity of endogenous peroxidase.
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