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Finally, the alignments blocks were extracted with ≥5 kb of the intervals.
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Conserved blocks were extracted using Gblocks with relaxed parameters (Castresana 2000).
There were actually 25 blocks, and 17 blocks were extracted using the proposed method, but 31 blocks were extracted by the DWSC method.
The raw data were extracted with MarkerView.
Lipids were extracted with 2∶1 chloroform/methanol.
Four individuals per block were extracted for each case, automatically excluding them from remaining blocks.
A core biopsy was taken from the tissue block and DNA was extracted with the Puregene DNA Isolation Kit (Gentra, Minneapolis, MN) as previously described [ 44, 45].
Total RNA was extracted with Trizol.
Total RNA was extracted with TRIzol (Sigma).
RNA was extracted with Trizol reagent (Invitrogen).
RNA was extracted with TRIZOL (Invitrogen).
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