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In total, the nucleotide and amino acid sequences of the mitochondrial protein-coding genes for 37 taxa were aligned and conserved blocks were extracted as described above.
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The four largest peaks among the segmented blocks are extracted as the parameters of the wall plane.
DNA from paraffin blocks was extracted as described (5 ) by using the DNeasy Tissue kit (QIAGEN, Hilden, Germany).
There were actually 25 blocks, and 17 blocks were extracted using the proposed method, but 31 blocks were extracted by the DWSC method.
All other bodily fluids need to be extracted as well.
Single particle tracks were extracted as above31.
Proteins were extracted as previously described by da Silva and coworkers13.
Pseudopodia were extracted as described by [37].
Lipids were extracted as described above.
Saponins were extracted as described in [17].
Four individuals per block were extracted for each case, automatically excluding them from remaining blocks.
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