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Accordingly, a total of 16 blocks were done for each session.
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Following PBS washes, blocking was done for 1.5 h at RT in blocking solution containing 5% serum/BSA with 0.1% Triton-X.
Blocking was done for 90 min at room temperature.
Antibody incubations were done for 1 hour in blocking buffer.
In this step, resource block scheduling is done for the direct link from the macro base station to the macro-users without allocating any resource blocks to the relay feeder link.
In the hybrid approach, dynamic process level profiling in the block layer is done for deciding the candidates for tiering to SSD.
Incubation in a peroxidase block (BioGenex) was done for 10 min. The coverslips were washed before a 30 min incubation in a 1 75 dilution of the primary anti-mouse proliferating cell nuclear antigen (PCNA) antibody (Chemicon International, Temecula, CA) and staining was done as previously described [ 44].
This means that one computation was done for blocks with SNP 1 100, 101 200 etc., and a second computation was done for blocks with SNP 1 50, 51 150 etc. and then the values of overlapping blocks were averaged to smooth out blocks of 50 SNP.
The same was done for the blocks in which the box was oriented upside down.
Blocking was done with Protein Block (Dako) for 20 minutes.
Endogenous peroxidase blocking was done with 1% H2O2 for 20 minutes at room temperature.
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