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Haplotype blocks were determined using a solid spine of LD (D′ = 1).
Haplotype blocks were determined using the algorithm of Gabriel et al [ 33].
The linkage disequilibrium blocks were determined using data from HapMap data release #16c.1, June 2005, on NCBI B34 assembly, dbSNP b124.
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Before each measurement, the height of the stack of blocks was determined using a ruler, due to small differences in the exact dimensions among gel blocks.
In the load spectrum development, the number of marker load cycles in a load block is estimated through a crack growth equation including the crack closure effect, while the interval of marker load blocks is determined using the crack length measured in a pilot test.
The orientation of the cutting blocks was determined using the navigation system, and after bone resection the cutting planes were documented.
The position and orientation of the zebrafish within the block was determined using secondary electron imaging mode (SEI, Everhardt Thornely Detector), whereas atomic number contrast of osmium within the tissue was imaged using a solid-state backscattered electron (BSE) detector [5], [3].
Block boundaries were determined using the criterion of Gabriel et al (2002).
The co-linear blocks of the 3 genomes were determined using BLASTn, and the alignment within each of the blocks was based on the Mauve method [63], with a seed length set to 11.
The longest non-overlapping haplotype blocks with between 3 and 20 SNPs were determined using the block definition of Gabriel et al. (2002).
Optimal transfection conditions for G401 F22 cells were determined using BLOCK-iT fluorescent oligo (Invitrogen).
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