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For hyphal autolysis assay, small agar blocks were cut from the edge of 4-day-old cultures and placed onto CM medium with 1 M sorbitol and cultured in the dark at 28°C for two weeks.
Blocks were cut from various cortical and subcortical regions, embedded in paraffin, sectioned, and then stained with hematoxylin and eosin.
After incubation, 5 × 5 mm diameter blocks were cut from the advancing edges of the culture and placed on top of a 2% water agar plates.
The tissue blocks were cut from each region and embedded in paraffin wax, from which 5 mm sections were prepared, mounted, and stained with hematoxylin-eosin.
Briefly, 4 × 4 mm agar blocks were cut from the above media and mixed with 1 drop of lactophenol cotton blue (phenol 20 mL, lactic acid 20 mL, glycerol 40 mL, and distilled water 20 mL).
The stained heart was left in Bouin's solution for 5 7 days, after which 2-mm-thick equatorial rings were cut through the ventricles and small transmural blocks were cut from the lateral LV free wall of these rings (53).
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The brown and grey office blocks are cut from the picture.
All pieces within each block were cut from the same plant when possible, and no individual plant was used in more than one block.
The resulting raw-data-slices as well as the extant material are stored at the Natural History Museum of Vienna (NHMW-2012/0133/0480-550). A 150×45×140 mm block was cut from the ammonoid-bearing limestone bed of Aşağiyaylabel, and for stabilization, set into concrete (Fig. 2B).
For paraffin sections, several 5-µm sections from paraffin blocks were cut and deparaffinized with xylene.
Serial 7 μm cross-sections from heart blocks were cut and fixed to coated slides.
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