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Resin blocks were cut by a hacksaw and, consequently, manually polished on abrasive foils with decreasing grain size.
These blocks were cut by a freezing microtome into 50 µm-thick serial coronal sections.
Serial sections (4 µm) of the paraffin blocks were cut by a microtome and mounted on silane-coated slides.
For animal studies, mice were sacrificed and paraformaldehyde-fixed tissue blocks were cut by vibratome at 40 µM thickness, followed by post-fixation processing and analysis as described above.
The paraffin blocks were cut by a microtome into 4 mm sections, which were subsequently stained with haematoxylin and eosin.
Blocks were cut by a Reichert Ultracut S ultramicrotome, thus obtaining ultrathin sections (70 nm) which were collected on nickel grids.
Similar(53)
One set of these blocks was cut by a freezing microtome into 50-µm thick serial coronal sections.
The tissue blocks were cut at 4 µm thickness by means of a Leica Rotary Microtome (Model 2165) and processed for Hematoxylin and Eosin (H&E) staining, as described before [34].
Corresponding formalin-fixed, paraffin-embedded tumour tissue blocks were cut, deparaffinised and rehydrated, followed by EDTA antigen retrieval, using standard anatomical pathology procedures.
The paraffin blocks were cut in 5 micrometer (μm) thicknesses by a microtome (Leica RM2255, Japan) and placed onto numbered slides.
Briefly, the tissue blocks were cut into 5 μm-thick sections, followed by deparaffinization and rehydration.
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