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For immunohistochemical analysis frozen lumbar spinal cord (L4 L6) or brainstem blocks were cut at 10 μm thickness using a cryostat (Leica).
Paraffin blocks were cut at 3 μm for immunohistochemistry.
The tissue blocks were cut at 4 µm thickness by means of a Leica Rotary Microtome (Model 2165) and processed for Hematoxylin and Eosin (H&E) staining, as described before [34].
Tissue blocks were cut at a thickness of 5-μm.
The blocks were cut at 5 μm sections.
Paraffin blocks were cut at 4-μm thickness, then fixed on glass slides.
Similar(43)
Sections from each tumour block were cut at 4 μm.
Each paraffin block was cut at a thickness of 10 μm into 5 slices.
The tissue blocks were cut serially at 50 μm thick sections.
The remaining, larger tissue blocks were cut coronally (at 50 μm) in a repeating series of 4 sections.
Blocks were cut conventionally at 3-4 μm thickness, dried and deparaffinized in xylene for 3 times, every time 15 minutes, and then rehydrated in graded ethanol.
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