Tumor blocks were collected for 828 patients.
All data were collected on-line.
Information on hormone receptor status was available for 148 of the 201 patients from whom tissue blocks were collected.
For 251/273 cases (92%) paraffin blocks were collected.
Tissue slices were collected on thin blocks of Polydimethylsiloxane (Sylgard 184; Dow-Corning, Midland, MI) and held in position using a plastic-framed soft mesh.
After polymerization, blocks were removed and trimmed, ultrathin sections were collected on formvar coated grids, and samples were imaged on a JEOL JEM1230 transmission electron microscope.
Previously identified HEK293 cells were located on the block face, and 70-nm serial sections were collected on Formvar-coated slot grids, post-stained with lead citrate, and imaged with an FEI Tecnai 20 microscope (FEI, Hillsboro, OR, USA), equipped with an Olympus-SIS Morada CCD camera (Olympus, Tokyo, Japan) and item software, or with a CM 120 Bio-Twin microscope (Philips).
To ensure the encapsulant was effectively blocking the release of PCBs into classrooms, wipe samples were collected on the encapsulated surfaces periodically during the longitudinal study.
During testing blocks, subject's responses as to whether sound and gesture match were collected on the forty one point visual analogue scale, (−20∶20).
The embedded blocks were then sectioned on an ultramicrotome with a diamond knife, and ultrathin sections were collected on collodion-coated copper grids.
