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Linkage disequilibrium (LD) and haplotype blocks were assessed using the Haploview programme (http://www.broad.mit.edu/mpg/haploview/) applying default parameters according to Gabriel et al [34].
Whole chromosome comparisons of larger orthologous blocks were assessed using the Cinteny server [ 42] and Roundup database [ 43].
Preliminary analyses revealed that d-prime values for block 8 and all subsequent blocks violated normality, thus analysis of the effects of task and stimulus pair type across the first 7 blocks were assessed using a 3 (task: novel-AV, verbal-AV, shape-AV) × 7 (block: 1, 2, 3, 4, 5, 6, 7) repeated measures ANOVA.
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Diversity of chemical structures for the styryl molecules and for their chemical building blocks was assessed using Cactvs bit strings which represent chemical structures as 881- dimensional binary sequences.
The level of sensory block was assessed using the pinprick test (1 = hypoalgesia; 2 = analgesia; 3 = analgesia plus hypoesthesia; and 4 = anesthesia) using a 22 G blunt hypodermal needle.
The level of motor block was assessed using the modified Bromage scale (0 = no motor block, able to flex hips, knees and ankles; 1 = just able to flex knees, unable to extend legs; 2 = able to move ankles, unable to flex knees; 3 = unable to flex ankles, knees or hips, complete motor block).
When the peak sensory block was determined, the degree of motor block was assessed using a modified Bromage Scale (in which 0 = no paralysis; 1 = unable to raise extended leg; 2 = unable to flex knee; 3 = unable to flex ankle) [ 16].
Synteny block assignments were assessed using BLOCKON within the LINKMFEX package.
Negative controls were assessed using normal serum (Ultra V Block, Laboratory Vision) as the primary antibody.
Thus, verbal memory abilities were assessed using California Verbal Learning Test and Digit Span and Rey-Osterrieth Corsiex Figure and Corsi Blocks was used to assess visual spatial memory abilities.
Time trends were assessed using birth cohorts.
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