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For the remainder, paraffin blocks were acquired from the archives and ER status ascertained for the purposes of this analysis.
Formalin-fixed, paraffin-embedded (FFPE) tumour blocks or unstained sections from FFPE tumour blocks were acquired from anatomical pathology laboratories performing the diagnosis and sent to the Peter MacCallum Cancer Centre Pathology department.
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Spectra were acquired in blocks of 4.56 min. Three blocks were acquired at baseline, four blocks during tDCS, and three blocks post-tDCS.
For each rf-tuning frequency of the proton channel, 8192 noise blocks were acquired.
Human FFPE block samples were acquired from various external academic sources in compliance with Pfizer's human tissue policy and approval by the Institutional Review Board of the source institution.
For evaluation of candidate biomarker antibodies, FFPE human prostate cancer tissue blocks with limited clinical annotation were acquired from other commercial sources.
Serial 4 μm sections were acquired from each paraffin block and stained with HE and PAS.
For each tumour 2 or 3 core samples of tumour tissue were acquired from the donor block.
For each case, 2 core samples of hepatoma tissue were acquired from a donor paraffin block provided by the Pathology Diagnosis Department of Zhongshan Hospital.
Data were acquired from six sedated adult males using a standard block design.
Images were acquired from 6 independent specimens (N = 6); 15 20 synapses were evaluated in each block (total number of spines: control, n = 112; treated, n = 94).
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