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Total RNA was extracted from frozen OCT blocks using the RNeasy® Lipid Tissue Mini Kit according to manufacturer instructions (Qiagen, Germantown, MD).
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Following homogenization, RNA was isolated using the RNeasy® Mini kit.
RNA was isolated from the total hypothalamic block using the RNeasy midi RNA isolation kit (Qiagen, Venlo, The Netherlands) and quantified by Nanodrop spectrophotometer ND-10000, Nanodrop Technologies, Wilmington, DE, USA).
RNA extraction was performed on two 10-μm sections taken from each of the tumor blocks by using the RNeasy FFPE RNA Isolation kit (Qiagen, Crawley, UK), according to the manufacturer's recommendations, except for an initial incubation in xylene (30 minutes, 37°C) for complete removal of paraffin.
Total RNA was extracted from archival tumor blocks or slides using the RNeasy FFPE kit (Catalog #73504 – Qiagen, Valencia, California) and fresh liver biopsies preserved in RNA later using the RNeasy mini kit (Catalog #74106 Qiagen) according to the manufacturer's instructions.
RNA was isolated using the RNeasy kit (Qiagen, Hilden, Germany).
Total RNA was purified using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instruction.
Total RNA was isolated using the RNeasy Mini Kit (Qiagen) following the manufacturer's protocol.
Total cellular RNA was extracted using the RNeasy mini kit (QIAGEN) following the manufacturer's instruction.
RNA isolation was performed using the RNeasy Mini kit (Qiagen) with a modified protocol.
Total RNA was extracted using the RNeasy plus minikit (Quiagen, #74134) according to manufacturer's instruction.
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