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Temperature, pH and salinity were measured in all treatments (n = 9 per treatment across both blocks) using a pH meter (WTW Wissenschaftilich-TechnischeWerkstätten GmbG P4) and probe (WTW SenTix® 41 pH electrode; precision ± 0.01 pH units).
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The pH was read using a pH meter.
The solution pH was measured using a pH meter PHM82.
The pH of filtrate was recorded using a pH meter.
The pH was then measured using a pH probe.
The pH was measured using a pH meter.
The water reaction (pH) was determined using a pH meter (pH meter CG 817).
Medium pH was measured using a pH electrode/meter (Horiba).
Spontaneous and adjusted pH was quantified using a pH electrode and pH test strips.
Antigen retrieval was achieved by microwaving sections for 20 min in 10 m M citrate buffer pH 6.0, and endogenous biotin activity was blocked using a biotin blocking kit (Vector Laboratories Ltd ,Peterborough, UK) to prevent non-specific background staining.
CXCR4 was then blocked using a specific monoclonal blocking antibody.
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