For structural analysis, thin sections – a few microns thick – are cut from a cell or tissue block using a microtome, mounted on a microscope slide, deparaffinized and finally cover-slipped using a mounting medium.
Sections 200 μm thick were cut from the polymerised blocks, using a sawing microtome (Sawing microtome Leica SP 1600) and mounted on acrylglas slides (Perspex GS Acrylglas PMMA/Opal 1013, Wachendorf AG, Basel, Switzerland).
A series of transverse sections 10-μm-thick were obtained from the sample blocks using a Minot rotary microtome.
Serial sections of 3 μm were prepared from paraffin blocks using a high-precision microtome and dried overnight at 37°C (Histology core facility, University Medical Center, Mainz).
Specimens were embedded in paraffin (Thermo Fisher Scientific GmbH, Dreieich, Germany), and cross sections of 10 μm thickness were obtained from the cured specimen blocks using a Leica RM2055 microtome (Leica, Nussloch, Germany).
Transverse sections approximately 1-µm thick were cut from the plastic blocks using a Reichert Ultracut E microtome, stained with 0.1% toluidine blue, and analyzed at 10× or 16× magnification under bright-field illumination.
Sections (4 μm thick) were cut from the FFPE Hela cell pellet block using a standard microtome, placed on SuperFrost®Plus glass slides (Menzler-gläser) and incubated for 45 min at 65°C.
Testicular cross sections fixed in 10%% formaldehyde solution then embedded into paraffin wax blocks and cut using a microtome.
Cross section of colon tissues were fixed in 10% formaldehyde solution then embedded into paraffin wax blocks and cut using a microtome.
Representative 4 μm sections of tissue blocks were cut using a microtome, mounted onto poly-l-lysine-coated slides and dried overnight at 37 °C.
