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The areas of interest were marked on the wax blocks using a marker pen and 0.6 mm diameter cores of tumour or normal breast tissue were removed and inserted into a pre-cored hole in a recipient paraffin block.
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We identified haplotype blocks using a feature available in the software, using the default parameters maximum length of 160 kb and maximum of 30 markers per block.
Using a marker pen, the corresponding region was circled on the archival 'donor' paraffin block.
Using a marker, trace patterns for the knife, fork, spoon and plates on construction paper.
Draw on a collar using a marker.
Add some details using a marker.
Sketch the actual lines using a marker.
Draw in the eyes using a marker.
CXCR4 was then blocked using a specific monoclonal blocking antibody.
Endogenous biotin was blocked using an endogenous biotin blocking kit.
Thus experiments were designed herein for large-scale comparative genetic mapping of melon and cucumber to identify syntenic blocks using microsatellite markers.
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