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Benign and malignant tissue specimens from a consecutive series of 448 patients undergoing radical prostatectomy for localized prostate cancer at Malmö University Hospital, Sweden, between 1988 and 2003 were mounted in 17 tissue microarray blocks using a manual tissue arrayer (Beecher Inc ,Wisconsin WI) as previously described[39].
Core needle biopsies were retrieved from the original tumor blocks using a manual arrayer (Beecher Instruments, Sun Prairie, WI) and positioned in a recipient paraffin array block.
Tissue microarrays (TMAs) were constructed from archival formalin-fixed, paraffin-embedded tissue blocks using a manual tissue arrayer (Quick-Ray Manual Tissue Microarrayer, Unitma Co. Ltd ,Seoul, Korea).
Two cores of 1.0 mm in diameter were mounted into the recipient TMA blocks using a manual arraying device (MTA-1, Beecher Inc, WI, USA).
Three different tissue cores within a single tumor were arrayed from formalin-fixed, paraffin-embedded tissue blocks using a manual device (Beecher Instruments, Sun Prairie, WI, USA).
Needle core-biopsies (0.6 mm) from the corresponding areas on the paraffin-embedded tumour blocks were then placed at pre-specified coordinates in recipient paraffin array blocks using a manual tissue arrayer (Beecher Instruments, Sun Prarie, WI, USA).
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Briefly, two 0.6 mm diameter tissue cores were collected from each tumour block and arranged in a recipient block using a manual tissue arrayer (Beecher Inc., Sun Prairie, WI).
For each tumor, three core biopsies 1 mm in diameter were then taken from selected areas of donor blocks and arrayed into a recipient paraffin block using a manual tissue arrayer (Beecher instruments, Sun Prairie, USA).
Briefly, the TMA was composed of three 0.6 mm cores from each of the tumor specimen assembled in a recipient paraffin block using a manual device from Beecher Instruments, Silver Spring, USA.
Core samples were removed (1.0 mm diameter) from each tumour and rearranged on empty paraffin-blocks using a manual tissue microarray device (BEECHER MTA II; Beecher Instruments, Inc., Sun Prairie, Wisconsin, USA).
Three cores per patient with a diameter of 0.8 mm were chosen and transferred to a paraffin block using a manual arrayer (Beecher Instruments, Sun Prairie, WI).
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