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Blank and negative controls, which were located in all blocks of the array, were used to confirm the stability of the experiment.
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We assumed that each group of 4 blocks was equivalent in average signal intensity and range to the next group of 4 blocks on the array.
The thickness of the 2D nanostructure, the building block of the arrays, has significant influence on pseudocapacitive properties of the arrays, thus their thickness optimization strategy is of great importance to the development of these 2D metal oxide nanostructure arrays, which is eagerly expected but rarely reported.
Blocking of the arrays was in 50 mM ethanolamine, 0.1 M Tris, pH 9.0, 0.1% SDS, at 50°C for 20 min, according to the manufacturer's protocol.
Triplicates of sense and antisense amino-modified β-lactamase PCR products were also deposited on each of the blocks of the cDNA array to monitor the processing of double-stranded DNA to single-stranded (ssDNA).
20 After the construction of the array block, all the tissue blocks were cut with a microtome to 4 μm and affixed to the slide.
Four μm thick sections of the Array blocks were stained with Ki-67.
However, only weak autocorrelation signals with no periodic peaks were observed when up to eight dispersed subarray blocks deviated from the rest of the array.
Following the construction of the array block, 5 μm sections were cut with a microtome.
Four μm thick sections of the Array block were stained for Ki-67.
After construction of the array block, multiple 5-μm sections were cut with a microtome and placed on polylysine-coated slides.
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