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Small blocks of sections with anterograde, NR1 or mGluR1a and CB or PV label were cut under a dissecting microscope, postfixed in 1% osmium tetroxide with 1.5% potassium ferrocyanide in PB, washed in buffer (PB) and water, and dehydrated in an ascending series of alcohols.
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To improve antibody penetration of the tissue, the sections were incubated for 1 h in 0.2% Triton X-100, followed by blocking of sections with 5% goat serum in PBS to prevent non-specific binding.
Blocking of sections was done with PBS/0.3% Tween-20/2 Tween-20/2inally incubated for 1 h in the presence of anti-α-tubulin DM1-A (Sigma, Missouri, USA) (1∶100), as a primary antibody and with anti-mouse IgG conjugated to FITC (ZYMED, California, USA) as secondary antibody (1∶30).
Those who are not in the squares and yards block off sections of the street with slabs of concrete and sleep right on the pavement.
From the paraffin blocks, pairs of sections were cut at 50-μm intervals until complete sectioning of the nodes was achieved.
Metal barriers block off sections of a crumbling walkway to keep tourists from falling in.
This block consisted of sections of varying densities to simulate both the trabecular and cortical bone comprising severely osteoporotic vertebral bodies.
By using personal family photographs, she purposely blocks out sections of each image with thread turning them more into objects, creating a form of what she calls "emptiness".
Blocks of coronally sectioned post-mortem STG tissue (1 cm thick) were sourced from the NSW Tissue Resource Centre, The University of Sydney, Australia.
In its ruling today, the court did not strike down the website blocking powers of Section 66A, however, according to DNA.
Cafes and stores were open in the central area, but blasted husks of buildings still line block after block of large sections of the city.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com