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Formalin-fixed paraffin-embedded tissue blocks from each patient were cut at 4-μm thickness.
To facilitate the screening of multiple tissue blocks from each patient, tissue arrays were prepared.
One or two representative tumor blocks from each patient were examined by immunohistochemistry.
Each tissue microarray (TMA) block contained a single 0.6 mm core sampled from representative paraffin blocks from each patient.
Haematoxylin and eosin (H&E -stained tissue sections from all tumour blocks from eacH&E -stainedre examined.
Ultra-thin sections from at least three small blocks from each patient were stained with uranyl acetate and lead citrate.
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In short, one 0.6 mm core was taken from a representative tissue block from each patient.
One representative block from each patient was sectioned at 5 μm and stained with the primary antibody.
FFPE tumour blocks were reviewed for quality and tumour content, and a single representative tumour block from each patient, containing at least ⩾60% of neoplastic cells, was selected.
For the current research project, the participating pathologists were asked to provide a representative formalin-fixed, paraffin-embedded tumour block from each patient.
The appropriate paraffin-embedded specimen blocks for each patient were obtained from the Pathology Department.
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