Tissue blocks embedding a representative sample of the tumor were used.
(iv) Immunogold staining Thin sections of the Eponate 12™ resin blocks embedded with tissue or RSE cells with E. coli O157 were cut 70 nm thick on the Leica UC7 ultratome (Leica).
Proper alignment of the rotary microtome object holder with respect to the block minimized excessive facing of the tissue, and blocks embedded with the mucosal surface oriented down were efficiently cut and produced more uniform paraffin ribbons.
The tissue blocks were embedded in Tissue-Tek OCT compound and quickly frozen in dry ice acetone.
The adipose tissue block embedded in paraffin was sectioned at 5 μm to measure the adipocyte area.
For each case three tissue cores (1.5-mm diameter) from the selected representative tumor areas were punched out of the sample tissue blocks and embedded into a new paraffin array block using a tissue microarrayer (Beecher Instruments, Woodland, CA).
For each case, tumour tissue cores (1.5 mm diameter) from the selected representative tumour areas were punched out of the sample tissue blocks and embedded into a new paraffin array block using a tissue microarrayer (Beecher Instruments, Woodland, CA, USA).
Each brain block was embedded in Tissue-Tek O.C.T. Compound (Sakura Finetek Japan Co., Ltd) and frozen on liquid nitrogen and stored at −80 °C until use.
TMAs are produced by removing cylindrical cores of tissue from up to donor paraffin blocks and embedding these into a single recipient paraffin block at set array coordinates.
After micro-CT scans, the extracted bone samples were fixed in a neutral 10% formaldehyde solution, and the tissue blocks were embedded in a resin for histological analysis.
Serial block face scanning electron microscopy (SBF-SEM) images a resin embedded tissue block surface and uses an inbuilt diamond knife to remove a thin layer of resin embedded tissue (70-150 nm) prior to re-imaging.
