Sentence examples for blocking was performed using from inspiring English sources
Endogenous peroxidases were deleted by incubation with 0.9% hydrogen peroxide for 15 min at room temperature (RT) and blocking was performed using 3% BSA for 1 h at RT. Antibodies were then applied in an optimal concentration overnight in a wet chamber.
Briefly, antigen retrieval was performed in 50 mM Tris buffer (pH 9.0) for 20 minutes, then blocking was performed using 10% normal goat serum diluted with PBS and 0.05% Tween-20 (PBS-T) for 1 hour at room temperature.
Additional blocking was performed using Biotin Blocking System (DAKO Carpintera, CA, USA).
Serum blocking was performed using 10% normal rabbit serum for 30 min at room temperature.
After rinsing, blocking was performed using sheep serum and reacting with each primary antibody.
Avidin/Biotin blocking was performed using a kit from Vector Laboratories (Burlingame, CA, USA).
Femoral nerve block was performed using a linear ultrasound probe (HFL50 15–6 MHz; SonoSite Inc., Bothell, WA, USA) and a 5-cm, 18-gauge Contiplex Tuohy needle (B-Braun Ltd., Tochigi, Japan) [2].
We performed a study to determine, using ultrasonography, the actual location of the LA when TAP block was performed using landmark-based technique.
Thereafter, a paravertebral block was performed using lidocaine (Xylocaine, AstraZeneca, Brussels, Belgium), and a full thickness third-degree burn injury was inflicted on the flanks (n = 12) (15 20 % body surface area; painless by itself as cutaneous nerves for sensation of pain are destroyed).
Identification of homologous blocks was performed using i-ADHoRe v2.1.
