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First, blocking the outputs of α/β core neurons during consolidation stage but not retrieval stage specifically impairs 24-h aversive LTM (Huang et al., 2012).
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In a previous study by Bray and Amrein [9], blocking the output of Gr68a neurons caused reduced courtship levels, bout initiation rate and copulation success when intact w1118 females were used as the courtship object in a 300 mm2 chamber with room light.
Blocking the output of PPL1-γ1pedc dopamineuronsneurons (MB438B) also resulted in a strong defect.
Blocking the output of all of the αβ neurons, or subdivisions within αβ, for extended periods after training disrupted appetitive and aversive LTM [121].
Blocking the output of the γ lobe neurons as well as the entire KC population impaired both appetitive and aversive visual memories.
DOI: http://dx.doi.org/10.7554/eLife.02395.015 Blocking the output of α′/β′ neurons or α/β neurons did not significantly reduce the performance compared to the controls, although MB008B-GAL4/UAS-shi MB008B-GAL4/UAS-shi MB008B-GAL4/UAS-shiirments1
To test this possibility, we blocked the output of the α/β KCs with Shits1.
(A and B ) Scheme of the temperature shift to block the output of corresponding neurons during training (A ) or test (B ).
(B and C ) Scheme of the temperature shift to block the output of corresponding neurons during training (B ) or test (C ).
In response to the reviewer's suggestion, we blocked the output of MBON-α1 and α/β KCs during early consolidation.
We transiently blocked the output of a broad population of KCs using MB010B-GAL4 (Bräcket et al., 2013) and MB247-GAL4 and found requirement for KCs in memory acquisition and retrieval.
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