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After blocking, the membrane was incubated overnight with rabbit polyclonal HeV G antisera (Dr. Christopher Broder, Uniformed Services University, Bethesda, MD) at a dilution of 1 1000 in antibody diluent (1% bovine serum albumin (BSA) in PBS with 0.1%Tween-20Tween-20

After blocking, the membrane was incubated with appropriate dilutions of primary antibody and secondary antibodies (Jackson ImmunoResearch Labs).

After blocking, the membrane was incubated with mouse anti-human PLK1 antibody at 1 1,000 at 4°C overnight followed by incubation with goat-anti-mouse IgG antibody at 1 2,000 for 1 h at room temperature.

After blocking, the membrane was incubated with anti-FLAG M2 antibody (Sigma) followed by appropriate secondary antibodies conjugated with horseradish peroxidase (HRP).

After blocking, the membrane was probed with serum (diluted from 1∶200 to 1∶1000) and CSF (dilution of 1∶ 10 or 1∶20).

After blocking, the membrane was probed with a primary antibody to either MITF (Zymed; 1∶1000), CDK2 (Santa Cruz; 1∶2000), BCL2 (Santa Cruz; 1∶1000), MLANA (Santa Cruz; 1∶5000).

After blocking, the membrane is incubated with primary antibodies and secondary antibodies.

After blocking, the membranes were incubated sequentially with the appropriate diluted primary and secondary antibodies.

After blocking, the membranes were incubated overnight at 4 °C with rabbit anti-human caspase-3/caspase-9 monoclonantibodiesies purchased from Boster Biological Engineering Co. (Wuhan, China).

After blocking, the membranes were incubated with primary antibodies (anti HO-1, Stressgen; anti-Bcl-xL, anti-cleaved caspase-3, Cell Signaling, Danvers, MA, USA).

After blocking, the membranes were probed with polyclonal CBS antibody(1∶2000 dilution) or monoclonal SUMO-1 antibody (1∶4000 dilution).