Sentence examples for blocking steps were performed from inspiring English sources

Antibodies used in double-labelling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Additional blocking steps were performed using Super Block and Mouse-to-Mouse Mouse-to-Mouse Mouse-to-Mouse the manufacturer instructions (ScyTek LaBlockinges).

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal sera of the host species from which the respective secondary antibodies were derived.

Dose response curves relating the magnitude of the absorption at 405 nm to peptide concentrations used in the assay plate coating step are found in Figure 2. Nunc Maxisorp assay plates were coated with aqueous solutions of peptide 1 at a concentration of 44.3 μM for 2 h at RT. Assay plate washing and blocking steps were performed as described above.

Blocking steps were performed as described earlier.

After hybridisation, stringency washes and a blocking step were performed.

A peroxidase blocking step was performed by incubating the sections for 10 minutes with 0.3% H2O2 in H2O followed by two PBS washes for 5 minutes each.

Blocking step was performed following instructions of Histostain-plus kit.

Blocking step was performed by incubation in 1% (wt/vol) bovine serum albumin or alternatively in 10% (vol/vol) goat serum.

A second blocking step was performed in 10% (v/v) normal serum (matched to secondary antibody species) in PBS for 30 min at 4°C with agitation.