Sentence examples for blocking steps were followed from inspiring English sources
After a 5-min wash in Tris-buffered saline (TBS), blocking steps were followed according to the manufacturer's guidelines using hydrogen peroxide solution and 10% casein.
Appropriate un-masking procedures using specific treatments, including hyaluronidase and pronase for collagens and citrate buffer solution for MMPs and TNF-α, followed by blocking steps were carried out.
After hybridisation, stringency washes and a blocking step were performed.
Biotin blocking step was repeated once followed by washes with 1× TBS.
Blocking step was performed following instructions of Histostain-plus kit.
A second 30 minute blocking step was applied in blocking buffer without Triton-X-100, followed by a 2 hour incubation with the secondary antibodies diluted in blocking buffer without Triton-X-100.
A peroxidase blocking step was performed by incubating the sections for 10 minutes with 0.3% H2O2 in H2O followed by two PBS washes for 5 minutes each.
The secondary blocking step was omitted.
This blocking step was repeated one more time.
Blocking steps were performed following SG substrate color development.
