Sentence examples for blocking steps were carried from inspiring English sources

Appropriate un-masking procedures using specific treatments, including hyaluronidase and pronase for collagens and citrate buffer solution for MMPs and TNF-α, followed by blocking steps were carried out.

All steps except the blocking step were carried out in 100 µl volumes and at room temperature except for ELISAs involving dengue virus which were done at 50 µl volumes.

An initial blocking step was carried out, for preventing any non-specific binding of CBMs, by incubating all samples in phosphate-buffered saline (PBS) (pH 7.4) containing 5% (w/v) ovalbumin.

Additional blocking steps were performed using Super Block and Mouse-to-Mouse Mouse-to-Mouse Mouse-to-Mouse the manufacturer instructions (ScyTek LaBlockinges).

Following the blocking step, all incubation steps were carried out at room temperature on a moving platform with the membranes being washed thoroughly in 3 × 15 min washes after each step.

All blocking, antibody incubation and wash steps were carried out with gentle agitation.

All steps were carried out at 4-8°C 4-8°C

All incubation steps were carried out at room temperature.

All the following steps were carried out at room temperature.

All subsequent incubation steps were carried out in the dark.

All incubation steps were carried out in a humidified chamber.