Sentence examples for blocking steps were applied from inspiring English sources

While extensive washing and blocking steps were applied, GTPase binding was determined by immunodetection using 5×His-specific antibodies (Qiagen) and TMB Thermo Scientificc) by a colorimetric quantification.

A second 30 minute blocking step was applied in blocking buffer without Triton-X-100, followed by a 2 hour incubation with the secondary antibodies diluted in blocking buffer without Triton-X-100.

Antibodies used in double-labelling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal sera of the host species from which the respective secondary antibodies were derived.

Additional blocking steps were performed using Super Block and Mouse-to-Mouse Mouse-to-Mouse Mouse-to-Mouse the manufacturer instructions (ScyTek LaBlockinges).

Twenty steps were applied.

After hybridisation, stringency washes and a blocking step were performed.

In brief the following sequence of steps was applied for single staining: blocking endogenous peroxidase activity; anti-HHV-7 (clone 5E1); biotin-conjugated goat anti-mouse antibody (Dako); horseradish peroxidase-conjugated streptavidin; and for clone KR4 a TSA amplification step (Perkin Elmer) was applied.

Afterwards, two preprocessing steps are applied (step 2).

The secondary blocking step was omitted.