Sentence examples for blocking step was performed from inspiring English sources

A peroxidase blocking step was performed by incubating the sections for 10 minutes with 0.3% H2O2 in H2O followed by two PBS washes for 5 minutes each.

Blocking step was performed following instructions of Histostain-plus kit.

A second blocking step was performed in 10% (v/v) normal serum (matched to secondary antibody species) in PBS for 30 min at 4°C with agitation.

Blocking step was performed by incubation in 1% (wt/vol) bovine serum albumin or alternatively in 10% (vol/vol) goat serum.

A first blocking step was performed with 3% BSA/PBS solution for 30 min. Slides were then incubated for 1 hr at 37°C with the primary antibody rat anti-mouse CD31 (4 µg/ml) (clone MEC 13.3; Becton Dickinson, San Jose, CA, USA) or the isotype control (4 µg/ml) (clone eBR2a; eBioscience).

After hybridisation, stringency washes and a blocking step were performed.

Additional blocking steps were performed using Super Block and Mouse-to-Mouse Mouse-to-Mouse Mouse-to-Mouse the manufacturer instructions (ScyTek LaBlockinges).

Antibodies used in double-labelling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal sera of the host species from which the respective secondary antibodies were derived.

Dose response curves relating the magnitude of the absorption at 405 nm to peptide concentrations used in the assay plate coating step are found in Figure 2. Nunc Maxisorp assay plates were coated with aqueous solutions of peptide 1 at a concentration of 44.3 μM for 2 h at RT. Assay plate washing and blocking steps were performed as described above.