Sentence examples for blocking step was applied from inspiring English sources

A second 30 minute blocking step was applied in blocking buffer without Triton-X-100, followed by a 2 hour incubation with the secondary antibodies diluted in blocking buffer without Triton-X-100.

While extensive washing and blocking steps were applied, GTPase binding was determined by immunodetection using 5×His-specific antibodies (Qiagen) and TMB Thermo Scientificc) by a colorimetric quantification.

The secondary blocking step was omitted.

This blocking step was repeated one more time.

Blocking step was performed following instructions of Histostain-plus kit.

Each step was applied for five minutes.

After hybridisation, stringency washes and a blocking step were performed.

First, a preprocessing step is applied to the document to prepare it for the block localization algorithm.

In brief the following sequence of steps was applied for single staining: blocking endogenous peroxidase activity; anti-HHV-7 (clone 5E1); biotin-conjugated goat anti-mouse antibody (Dako); horseradish peroxidase-conjugated streptavidin; and for clone KR4 a TSA amplification step (Perkin Elmer) was applied.

Antibodies used in double-labelling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.

Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal serum of host species from which respective secondary antibodies were derived.