Sentence examples for blocking solutions were used from inspiring English sources

Ionic blocking solutions were used to isolate capacitive currents.

Blocking solutions were used for washes at room temperature and secondary incubation at 4°C overnight followed by additional room temperature washes.

The primary antibody AbTGZ4 [18] was used at 1 5.000 dilution and a solution of 12 nm diameter colloidal gold-affinipure anti-mouse IgG (Jackson Immunoreserach) diluted at 1 30 in the blocking solution was used as the secondary antibody.

Thus, 100  μL of blocking solution was used as the negative control for the assay.

After Tris-buffered saline (TBS) washing, blocking solution was used (Protein Block Serum-Free, Ready to use, Dako).

For tissue treated with Dil-Lp, 2%BSA/0.1%% Tween-20 PBS blocking solution was used to preserve Dil localization.

The same blocking solution was used for incubation with primary antibodies overnight at 4°C under gentle agitation.

After washing twice with a washing buffer (0.05% Tween20 in PBS), the blocking solution was used for blocking nonspecific binding for two hours at room temperature.

After washing twice with a washing buffer (0.05% Tween20 in PBS), the blocking solution was used for blocking nonspecific binding for 2 hours at room temperature.

To detect and quantify expression of 27-hydroxylase, a 1 : 300 dilution of primary antibody in blocking solution was used overnight at 4°C. 1 : 500 dilutions of primary antibody were used for detection of ABCA1, ABCG1, and LXR- α.

To detect recombinant I329L, a primary rat monoclonal high-affinity antibody against HA diluted in blocking solution was used (1 h, RT), followed by a donkey affinity-purified anti-rat IgG antibody conjugated with AMCA (1 h, RT).