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Biotinylated Galanthus nivalis agglutinin (GNA, B-1246), agarose-bound GNA AL-12433), agarose-bound streptavidin (Sand010), and Carbo-Free Blocking Solution were obtained from Vector Lab.
Similar(59)
Biotin blocking solution was added, samples were incubated and centrifuged.
Ultrablock (blocking solution) was from Serotec (Raleigh, NC).
The excess blocking solution was removed with filter paper.
Primary antibody (in blocking solution) was incubated for 60 min and then washed three times with blocking solution.
Ionic blocking solutions were used to isolate capacitive currents.
We repeat the process of identifying blocking loops and forbidding non-tree edges until a valid solution is obtained.
In FE software, the eigenvalue solution is obtained using either Block Lanczos or the Subspace Method solvers.
A blue-colored solution was obtained at this stage.
A clear solution was obtained with MolRep.
From this, a first solution was obtained.
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