Sentence examples for blocking solution were applied from inspiring English sources

For visualization of MCETs, primary antibodies directed against human mast cell tryptase (clonal AA1, mouse, DAKO) and C. albicans antibody (mouse monoclonal, ProSci) diluted in blocking solution were applied overnight at 4 °C.

Secondary antibodies in blocking solution were applied for 1 h at room temperature (RT).

HRP-conjugated secondary antibodies (Jackson Research Laboratories , Inc in blocking solution were applied to the membrane after four TBST washes and incubated for 2 h at room temperature.

Antibodies (rabbit anti-MDGA2A 1:300, mouse anti-GFP (Roche) 1 200 in blocking solution) were applied and incubated ON at 4°C.

Then, rhodamine-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse IgG polyclonal antibodies (each 1 500 in blocking solution) were applied for 30 min, and the slides were observed using a fluorescence microscope (Leica Microsystems).

After washing, the respective secondary antibodies (goat anti-rat 488, goat anti-mouse 568; goat anti-mouse 488, goat anti-rabbit 568, all Molecular Probes (Eugene, OR, USA), 1/1000 in blocking solution) were applied for 2 h at room temperature.

After transfer and blocking with 5% skimmed milk in TBS, the primary antibody diluted in blocking solution was applied for 2 hours at 37°C.

The primary antibody diluted in blocking solution was applied for one hour, except E-cadherin which was stained overnight at 4°C.

After three washes in PBS (5 min each), the secondary antibody, diluted in blocking solution, was applied for 1 h (Alexa 488, goat anti rabbit, 1∶1000, Invitrogen, Karlsruhe, Germany).

Anti-cyclin D1 antibody (1/500 in blocking solution) was applied overnight at 4°C.

Anti-MyoVI antibody in blocking solution was applied overnight at 4°C.