Sentence examples for blocking solution were added from inspiring English sources

Primary antibodies diluted in blocking solution were added and incubated for ∼1 h at room temperature.

For IgG1 binding assays, anti-HA IgG1 in blocking solution were added to wells and incubated for 1 hour at room temperature, after which bound IgG1 was detected using a peroxidase-conjugated mouse anti-human IgG antibody (Jackson).

Cells on glass coverslips were fixed in 100% methanol for 5 min at −20°C, washed in PBS and incubated in blocking solution (1% FBS/PBS) for 30 min. Primary antibodies in blocking solution were added for 2 h at room temperature (RT): 1∶1000 rabbit α-GFP (Ab290, Abcam) or 1∶500 mouse α-γ-tubulin (GTU-88, Sigma).

After washing three times with TBST, secondary antibodies diluted in blocking solution were added and incubated for 1 hour at room temperature.

Following three washes of D-PBS, 50 μl/well of primary antibodies at 2 μg/ml in blocking solution were added for 1 h at room temperature (RT).

Next, 250 µL of anti-DIG-alkaline phosphatase F ab fragments (diluted 1∶5000 in 1% blocking solution) were added for 30 min at RT.

Biotin blocking solution was added, samples were incubated and centrifuged.

After three PBS washes, secondary antibody in blocking solution was added for 3 hr.

Goat serum blocking solution was added drop-wise and slices were incubated for 5 h.

Then serially diluted serum in blocking solution was added in duplicate and incubated 1 h at 37°C.

Then Streptavidin-dye conjugate SA-Atto647N (ATTO-TEC, Siegen, Germany) diluted 1 25 in 250 µl new blocking solution was added.