Sentence examples for blocking solution was washed from inspiring English sources

After standing 2 h at RT, the blocking solution was washed away by means of centrifugation.

The blocking solution was removed by washing with the binding buffer 1 and then with acetate buffer (0.1 M. pH 4, containing NaCl 0.5 M).

The blocking solution was drawn off and slides were washed three times with 2 mL milliQ water each time.

Excess blocking solution was removed by successive washing steps with PBS-Tween 0.05%.

A total of 250 µL scFv-Fc fusion protein with a final concentration of 2 µg/mL in blocking solution was incubated for 1.5 h and washed three times with PBS.

The blocking solution was removed, and wells were then washed three times with 100  μl of 150 mM NaCl and 25 mM Tris-Cl, pH 7.4, and containing 1 mg/ml BSA (washing buffer).

Coverslips were washed and incubated in 1X PBS pH 7.4 containing 5% FBS and 3% BSA (blocking solution) for at least 30 min. Primary antibody diluted in blocking solution was incubated for 30 60 min, the coverslips washed 3X with blocking buffer and then incubated for 30 60 min with secondary antibodies: Alexa Fluor 594 for mouse diluted 1 2000 in blocking buffer.

The coated wells were washed with blocking solution (R10S medium), fresh blocking solution was added to each well, and then the plate was incubated for 2 hours at room temperature.

Primary antibody (in blocking solution) was incubated for 60 min and then washed three times with blocking solution.

After Tris-buffered saline (TBS) washing, blocking solution was used (Protein Block Serum-Free, Ready to use, Dako).

The blocking solution was drained from the samples (without washing) and incubated over night in anti-MyoD1 mouse monoclonal antibody (1 : 200 dilution in PBS) at 4°C.