Sentence examples for blocking solution was used from inspiring English sources

The primary antibody AbTGZ4 [18] was used at 1 5.000 dilution and a solution of 12 nm diameter colloidal gold-affinipure anti-mouse IgG (Jackson Immunoreserach) diluted at 1 30 in the blocking solution was used as the secondary antibody.

Thus, 100  μL of blocking solution was used as the negative control for the assay.

After Tris-buffered saline (TBS) washing, blocking solution was used (Protein Block Serum-Free, Ready to use, Dako).

For tissue treated with Dil-Lp, 2%BSA/0.1%% Tween-20 PBS blocking solution was used to preserve Dil localization.

The same blocking solution was used for incubation with primary antibodies overnight at 4°C under gentle agitation.

After washing twice with a washing buffer (0.05% Tween20 in PBS), the blocking solution was used for blocking nonspecific binding for two hours at room temperature.

Ionic blocking solutions were used to isolate capacitive currents.

For membrane blocking, a 5% Top-Block solution was used.

A blocking step for 1 h using 5%BSA/5%% donkey serum (block solution) was used prior to incubation with the goat anti-cathepsin B or rat anti-lamp-1 antibodies in block solution for 1 h.

Blocking solutions were used for washes at room temperature and secondary incubation at 4°C overnight followed by additional room temperature washes.

The blocking solution was replaced with PBS and the plates were stored at −20°C until use.