Sentence examples for blocking solution was then from inspiring English sources
The anti-Sirt1 antibody in blocking solution was then added (1∶1,000) and incubated overnight at 4°C.
A volume of 100 μl of blocking solution was then added to each well and gently mixed for 30 seconds.
The blocking solution was then discarded and 100 μl A2L2 cells (2 × 10-1 × 10) was added to each well of the ELISPOT plate.
Alkaline Phosphatase-conjugated anti-digoxigenin antibody (Roche Biochemicals, Basel, Switzerland) in blocking solution was then added to slides and incubated overnight at 4°C in a humidified chamber.
Monovalent Fab fragments of goat anti-human IgM+IgG (Jackson Immunoresearch Laboratories, Inc ., in blocking solution, was then applied for secondary blocking.
Incubation with primary antibodies diluted in blocking solution was then carried out for 1 hr at room temperatures or overnight at 4°C.
Membranes were then washed with blocking solution, and appropriate secondary antibodies diluted in blocking solution were then applied for 1 h at room temperature.
The primary antibodies (against biotin and the protein partner to be tested) diluted in blocking solution were then added, and the mixture was incubated overnight at 4 °C.
The respective HRP (horseradish peroxidase -conjugated anti-IgG secondary antibodies (1:2000 for N- and C- terminal NDRG1, 1:1000 for peroxidase -conjugatedl diluted in blocking solution) were then incubated for 1 h at room temperature.
The blocking solution was removed, and wells were then washed three times with 100 μl of 150 mM NaCl and 25 mM Tris-Cl, pH 7.4, and containing 1 mg/ml BSA (washing buffer).
