Sentence examples for blocking solution was replaced from inspiring English sources

The following morning, blocking solution was replaced and fresh primary antibodies were applied at 1∶1000 NAIP (Abcam), 1∶3500 Actin (Sigma), and 1∶3500 HA (BAbCO) for one hour at room temperature under constant agitation.

The blocking solution was replaced with fresh solution containing mouse monoclonal βIII tubulin antibody (1∶2000; Promega, Madison, WI) plus rabbit polyclonal glial fibrillary acidic protein (GFAP) antibody (1∶500; DakoCytomation, Carpinteria, CA) and incubated for 60 minutes at room temperature.

Subsequently, non-specific binding was blocked with 10% normal goat serum in 0.05% Tween in PBS (PBST) for 30 min at RT after which the blocking solution was replaced by primary antibody solution diluted in 2% normal goat serum (NGS) in PBST (60 min at RT).

The blocking solution was replaced with fresh solution containing primary antibodies.

The blocking solution was replaced with PBS and the plates were stored at −20°C until use.

The blocking solution was replaced by 4 μg·mL−1 anti-myc Alexa647 antibody diluted in PBS for 1 h.

After fixation, they were rinsed twice with PBS and incubated with a blocking solution (5% FBS) and 0,2% Triton X-100 for 30 min. Afterwards the blocking solution was removed and replaced with fresh blocking containing the primary antibody (Anti-P-MSK antibody 1∶150, MBL International) and incubated overnight.

Then the blocking solution was removed and replaced by 100 μl of alkaline phosphatase-conjugated anti-dioxygenin-Fab fragment (Roche Diagnostics) diluted 1 1000 in 2% BSA.

Biotin blocking solution was added, samples were incubated and centrifuged.

Ultrablock (blocking solution) was from Serotec (Raleigh, NC).

Control sections in which the primary antibody solution was replaced by blocking solution did not show any signal in the seminiferous tubules (Fig. 2D).