Sentence examples for blocking solution was removed from inspiring English sources

Excess blocking solution was removed by successive washing steps with PBS-Tween 0.05%.

After fixation, they were rinsed twice with PBS and incubated with a blocking solution (5% FBS) and 0,2% Triton X-100 for 30 min. Afterwards the blocking solution was removed and replaced with fresh blocking containing the primary antibody (Anti-P-MSK antibody 1∶150, MBL International) and incubated overnight.

The excess blocking solution was removed with filter paper.

After the blocking solution was removed, 0, 1 or 10 ng/mL of FL-rTDP-43 were added to wells and incubated for 16 h at 4°C.

The blocking solution was removed by washing with the binding buffer 1 and then with acetate buffer (0.1 M. pH 4, containing NaCl 0.5 M).

Then the blocking solution was removed and replaced by 100 μl of alkaline phosphatase-conjugated anti-dioxygenin-Fab fragment (Roche Diagnostics) diluted 1 1000 in 2% BSA.

After the incubation, the Detector Block Solution was removed and the membrane strips were washed four times with Biotin Wash Solution 1× (KPL, Inc .. Immunoreactivity was determined by means of the ECL chemiluminescence reaction (KPL, Inc .. Mitochondrial extracts were prepared using the Mitochondrial Fractionation Kit (Active Motif).

The blocking solution was carefully removed and the denatured LNA probe (see previous section) 1 500 was added.

The blocking solution was replaced with PBS, the stamp was removed, and the slide was washed for 30 min in 100 ml PBS (gentle stirring).

Biotin blocking solution was added, samples were incubated and centrifuged.

Porous β-TCP scaffolds were immersed in aqueous solutions containing 0.5, 1.0, or 2.0 mass% gelatin for 30 s, taken out, and the redundant solution was removed by wiping the blocks with paper towels.