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Incubation with primary antibodies diluted in blocking solution was performed over night at 4°C.
Incubation of the primary antibody diluted in blocking solution was performed overnight, as shown in Fig. S9.
Incubation with primary antibody (serum samples) (1/50 in blocking solution) was performed at room temperature for 1 h, followed by 3 washing steps of 10 min each in PBS/0.1% Tween-20.
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Biotin blocking solution was added, samples were incubated and centrifuged.
Ultrablock (blocking solution) was from Serotec (Raleigh, NC).
The excess blocking solution was removed with filter paper.
Primary antibody (in blocking solution) was incubated for 60 min and then washed three times with blocking solution.
The patient was then turned to the supine position and iliac crest block, with 5 mL of the same local anaesthetic solution was performed [ 14].
However, the efficiency of block Gauss-Seidel becomes higher if the repetitive model solution is performed.
Ionic blocking solutions were used to isolate capacitive currents.
Next, blocking was performed with blocking solution for 30 min at room temperature (Roche) and rabbit immunoglobin fraction (Dako).
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