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After sealing of the microchip surface with the cover film, blocking solution was infused into each microchannel; and the microchip was left undisturbed for 1 hr at room temperature.
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Prewarmed Tyrode solution was infused into the intestinal lumen, the infusion rate being 0.5 ml min-1.
A continuous infusion of 3 mL.kg−1.h−1 of lactated Ringer's solution was infused throughout the experiment.
In the control animals, only Ringer's solution was infused.
Cardiac arrest was achieved with aortic cross-clamping, and glucose-insulin-potassium solution was infused.
The buffer solution was infused continuously to reach a liquid turnover of once a day.
Ringer's Lactated solution was infused throughout the experiment at 10 ml/kg/hr.
A total of 2350 mL bicarbonate Ringer's solution was infused.
with methanol (4 gm/kg), 12 h latter, Carbicarb solution was infused, following this enzyme was infused (i.v). in bolus.
The sample solution was infused via an Apollo IV electrospray source at 180 μL/h using a syringe pump.
The sample solution was infused via an Apollo II electrospray source at a 180 μL/h by a syringe pump.
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