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The blocking solution was drawn off and slides were washed three times with 2 mL milliQ water each time.
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Biotin blocking solution was added, samples were incubated and centrifuged.
Ultrablock (blocking solution) was from Serotec (Raleigh, NC).
The excess blocking solution was removed with filter paper.
Primary antibody (in blocking solution) was incubated for 60 min and then washed three times with blocking solution.
The blocking solution was replaced with fresh solution containing primary antibodies.
For the graphite blocks and carbon paper anodes from the MEC a simpler application procedure was used, where the latex was applied by dipping the blocks or paper into the latex, and excess solution was drawn off the anode with a laboratory wipe.
Ionic blocking solutions were used to isolate capacitive currents.
Blocking solution was removed and primary antibody in blocking solution applied at the required dilution.
Blocking solution was used to block the endogenous peroxidase activity.
For membrane blocking, a 5% Top-Block solution was used.
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