Sentence examples for blocking solution was discarded from inspiring English sources

After 40 minutes, the blocking solution was discarded and the membrane was incubated with a binding buffer, Solution F. After washing, the chemiluminescent substrate was incubated with the membrane for two to five minutes at room temperature.

The blocking solution was discarded and diluted mouse sera (1∶300; first antibody) in 3% NDS +0.4% Triton X-100 in 0.05 M PB was added and incubated O/N at RT.

The blocking solution was then discarded and 100 μl A2L2 cells (2 × 10-1 × 10) was added to each well of the ELISPOT plate.

The coating solution was discarded and blocking buffer (TBS (Tris buffer saline composed of Tris 3 g/l, NaCl 8 g/l, KCl 0.2 g/l adjusted to pH 7.4), 10 mM CaCl2, 0.05% Tween 20, 2% BSA) was added (1 hour incubation).

After 4 h incubation, unreduced MTT solution was discarded.

The supernatant solution was discarded and the precipitate preserved.

The solution was discarded and the beads were washed three times with nonreducing F-buffer.

The solution was discarded and the gels were dried in a speed vac for 30 minutes.

After cooling to RT the 0.1% SDS solution was discarded and the procedure was repeated twice.

Plates were incubated at 30°C in dark for one hour before the solution was discarded.

The solution was discarded.