Sentence examples for blocking solution was aspirated from inspiring English sources

The blocking solution was aspirated and cells were incubated in mouse anti-[LAMP-1] (Abcam #25630) diluted 1∶100 in 10% blocking solution for 1 h at room temperature.

The blocking solution was aspirated and sections were incubated overnight at 4°C in primary antibodies diluted in PBS with 0.3% Triton and 1% bovine serum albumin (BSA) (Sigma-Aldrich).

Excess blocking solution was aspirated and sections were incubated for 90 min at room temperature with 1 : 100 dilution of monoclonal goat anti CD31 PECAM1-M200) (Santa Cruz, Biotech, Santa Cruz, CA, USA).

To ensure efficient cell permeability, cells were washed with 250 μl of 0.2% triton × 100 in PBS, after which cells were blocked with 90% FCS in PBS containing 0.05% Tween 20 (Sigma, Israel) for 30 min. The blocking solution was aspirated and monoclonal mouse anti-M13 (1/100 dilution; GE healthcare, USA) was added for 1 h incubation following ×2 washes with 2% BSA in PBS.

After aspirating the coating solution, blocking solution was added to the wells for 2 hours and then the coated plates were washed three times.

The coating solution was aspirated off, and unoccupied binding sites on the plates were blocked with 2%% bovine serum albumin in PBS at 37 °C for 1 h.

Excess PLO solution was aspirated; then, the surface was rinsed with DPBS before use [27].

Subsequently, the solution was aspirated and the insoluble formazan product was solubilized with acidified iso-propanol.

Eventually, the staining solution was aspirated and traces of staining agent were removed by rinsing several times with PBS.

For electrospinning, the fibroin solution was aspirated with a 2-ml syringe having a diameter of 0.55 mm.

The suspension was centrifuged at 1200 rpm for 6 min, and the sodium citrate solution was aspirated.